human pro mmp 13 quantikine elisa kit  (R&D Systems)

Journal: Scientific Reports

Article Title: Cycloastragenol attenuates osteoarthritis by restoring chondrocyte senescence via the NRF2/NF-κB signaling axis

doi: 10.1038/s41598-026-43064-z

Figure Lengend Snippet: CAG attenuates oxidative stress-induced chondrocyte senescence. ( A ) Chemical structure of CAG. ( B ) Effects of CAG treatment for 24 h on the viability of chondrocytes were evaluated using the CCK-8 assay. n = 3. ( C ) Effects of CAG treatment for 48 h on the viability of chondrocytes were evaluated using the CCK-8 assay. n = 3. ( D – G ) Western blot analysis and quantification of senescence key markers (p53, CDKN1A, CDKN2A) in chondrocytes under CAG (50 μm) and TBHP (50 μm) treatments (See Supplementary Materials for original blots). n = 3. * p < 0.05; ** p < 0.01; *** p < 0.001. ( H , I ) Representative images and quantitative analysis of β-galactosidase staining. n = 3, bar = 50 μm. ( J , K ) Representative images and quantitative analysis of EdU staining. n = 3, bar = 50 μm. ( L – P ) ELISA quantification of IL-1β, IL-6, TNF-α, IL-17, and MMP13 levels in chondrocytes under different treatments. n = 3. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: Levels of SASP-associated inflammatory and matrix-degrading components (IL-1β, IL-6, IL-17, TNF-α, MMP13) were quantified using commercial ELISA kits (IL-1β: E-EL-R0012; IL-6: E-EL-R0015; IL-17: E-EL-R0566; TNF-α: E-EL-R2856; MMP13: E-EL-R0045 all from Elabscience Biotechnology) according to the manufacturers’ instructions.

Techniques: CCK-8 Assay, Western Blot, Staining, Enzyme-linked Immunosorbent Assay

Journal: The EMBO Journal

Article Title: TRPML1 suppresses pulmonary fibrosis by limiting collagen and elastin deposition

doi: 10.1038/s44318-026-00712-4

Figure Lengend Snippet: ( A ) UMAP plot showing annotated cell clusters. Major cell types are labeled and color-coded, including epithelial, immune, endothelial, and stromal populations. MMP expression was determined in 32 different cell types. ( B ) UMAP plots showing the expression of selected MMP genes (MMP10, 11, 15, 16, 17, 20, 21, 23, 24, 25, 27, 28) in single cells from mouse lung tissue (filtered air group, n = 9). Expression is color-coded by log-normalized expression levels, with darker shades indicating higher expression. ( C – E ) qRT-PCR data showing mRNA expression levels of Mmp1a, Mmp1b, Mmp2, Mmp3, Mmp9, Mmp12, Mmp13, Mmp14, and Mmp19 in pmLF, IMΦ or AMΦ (WT and Trpml1 −/− ). ( F ) qRT-PCR data showing mRNA expression levels of Timp1, Timp2, Timp3, Timp4 in pmLF and IMΦ (WT and Trpml1 −/− ). In all figures, each single dot corresponds to one biologically independent sample. Data were mean ± SEM. Statistical analysis for qRT-PCR data were performed with multiple t -test, corrected for multiple comparisons using the Holm–Šídák method. ( G – J ) Western Blot analysis of different MMPs in pmLF isolated from WT and Trpml1 −/− mice. Graphs show quantification of each MMP band normalized to ß-actin. Each single dot corresponds to cells isolated from one mouse. Data were mean ± SEM. Student’s t -test, unpaired, two-tailed. .

Article Snippet: MMP13 ELISA kit , Novus biologicals , NBP3-06930.

Techniques: Labeling, Expressing, Quantitative RT-PCR, Western Blot, Isolation, Two Tailed Test

Journal: The EMBO Journal

Article Title: TRPML1 suppresses pulmonary fibrosis by limiting collagen and elastin deposition

doi: 10.1038/s44318-026-00712-4

Figure Lengend Snippet: ( A ) UMAP plot showing annotated cell clusters. Major cell types are labeled and color-coded, including epithelial, immune, endothelial, and stromal populations. MMP expression was determined in 32 different cell types. ( B ) UMAP plots showing the expression of selected MMP genes (MMP10, 11, 15, 16, 17, 20, 21, 23, 24, 25, 27, 28) in single cells from mouse lung tissue (filtered air group, n = 9). Expression is color-coded by log-normalized expression levels, with darker shades indicating higher expression. ( C – E ) qRT-PCR data showing mRNA expression levels of Mmp1a, Mmp1b, Mmp2, Mmp3, Mmp9, Mmp12, Mmp13, Mmp14, and Mmp19 in pmLF, IMΦ or AMΦ (WT and Trpml1 −/− ). ( F ) qRT-PCR data showing mRNA expression levels of Timp1, Timp2, Timp3, Timp4 in pmLF and IMΦ (WT and Trpml1 −/− ). In all figures, each single dot corresponds to one biologically independent sample. Data were mean ± SEM. Statistical analysis for qRT-PCR data were performed with multiple t -test, corrected for multiple comparisons using the Holm–Šídák method. ( G – J ) Western Blot analysis of different MMPs in pmLF isolated from WT and Trpml1 −/− mice. Graphs show quantification of each MMP band normalized to ß-actin. Each single dot corresponds to cells isolated from one mouse. Data were mean ± SEM. Student’s t -test, unpaired, two-tailed. .

Article Snippet: The following ELISAs were used for the experiments: SP-A ELISA (NBP2-76693, Novus biologicals), Cathepsin K ELISA (NBP3-00426, Novus biologicals), TIMP1 ELISA (ab196265, Abcam), TIMP2 ELISA (ab227893, Abcam), MMP1 ELISA (NBP3-06885, Novus biologicals and ABIN6963621, Antibodies online), MMP2 ELISA (ab254516, Abcam), MMP3 ELISA (ab100731), MMP8 ELISA (ab206982, Abcam), MMP9 ELISA (MMPT90, R&D systems), MMP12 ELISA (ab213878, Abcam), MMP13 ELISA (NBP3-06930, Novus biologicals), MMP14 ELISA (NBP3-06941, Novus biologicals and ABIN6957687, Antibodies online), MMP19 ELISA (NBP3-06941, Novus biologicals), IL-17A ELISA (ab199081, Abcam), DESMOSINE ELISA (CSB-E14196m, Cusabio).

Techniques: Labeling, Expressing, Quantitative RT-PCR, Western Blot, Isolation, Two Tailed Test